作    者：蔣明, 等
影響因子：1.026
刊物名稱：Australasian Plant Pathology
出版年卷：2016, 45(3): 327-334

文章摘要：WRKYtranscription factors play an important role in plant growth, development and immunity. In our study, a WRKY family member gene designated BoWRKY6 was isolated from broccoli (Brassica oleracea var. italica), and its expression was induced by downy mildew (Hyaloperonospora parasitica). Five transgenic broccoli lines over-expressing BoWRKY6 driven by the CaMV 35S promotor were obtained by Agrobacterium tumefaciens mediated transformation, and they demonstrated significant increased resistance to downymildew, with resistant levels from low to very high. Real timequalitative PCR analysis indicated that expressions of both BoWRKY6 and the pathogenesis-related gene 1 (BoPR1) in transgenic plants were obviously higher than those inWT plants after H. parasitica treatment. Lines of BWK14 and BWK31 exhibited very high resistance to downy mildew, and may serve as promising candidate materials for broccoli molecular breeding in the near future.

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